ABSTRACT
Aims/objectives: The cognition about microbial population of activated sludge and their treatment potential will be very useful for industrial wastewater treatment plant operation. Methodology: In this study microbial population of activated sludge process that was used for pharmaceutical wastewater has been investigated. Sampling was done from return sludge line and after serial dilution 1500 plates were studied. Methods for separating the bacteria from wastewater was pour plate method. All bacterial samples were purified using nutrient Agar and Macconkey Agar culture. Bacteria were separated from return sludge line and classified into 3 groups after biochemical tests and morphological analysis, These include positive bacteria of Bacillus genus, Pseudomonas aeruginosa and Flavobacterium. Results: The biodegradability study on pharmaceutical effluent using identified cultures in laboratory scale showed that Bacillus spp. are the most efficient bacteria for organic matter degradation. Conclusion: Results of this study showed that providing a microbial bank of these spp. can be useful for resistant operation of activated sludge.
ABSTRACT
The purpose of this study was to determine antibacterial activity of essential oil of Satureja khuzestanica against Listeria monocytogenes [PTCC1295] and strains isolated from breast milk show that. In this descriptive-analytic study, Essence of leave's plant was extracted and identified its compounds and then carvacrol was isolated. Antibacterial activities were examined by agar dilution method against L. monocytogenes. Minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC] were carried out by micro dilution method. Then bacterial suspension injected the BALB/c mice. Forty-eight h after seeing the listeriosis disease signs were started the treatment. Ampicillin [10 micro g/disc] and trimethoprim [5 g] were used as controls. The results showed that the inhibitory zone diameter standard and essential oils for strains isolated species were respectively 59 and 50 mm. This amount was determined by carvacrol, respectively, 60 and 48 mm. Inhibition zone diameter measurements for standard strains of ampicillin and trimethoprim tedious strains, respectively, 21, 40, 18 and 33 mm, respectively. The minimum inhibitory concentration of essential oils, carvacrol and ampicillin than standard strains, respectively 1.56, 1.56 and 155x10[-8] micro g/mL and MBC 3.125, 3.125 and 125x10[-7] micro g/mL was determined by the ratio of the strain 3.125, 3.125 and 0.0062 micro g/mL and MBC was 6.25, 6.25 and 0.025 micro g/mL. This study showed that bacterial cleansing properties of essential oil of this plant have a strong and effective combination that is carvacrol